Imaris for Neuroscientists | Automatic Detection of Microtubules & Neurons in 2D, 3D and 4D | Bitplane
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Imaris for Neuroscientists

Imaris F1 Package is the perfect combination of tools for researchers working in various neuroscience disciplines. Imaris F1 enriches advanced 3D/4D visualization and analysis methods with Filament Tracer - best in class software for an automated detection of neurons including spines and other filamentous structures in 2D/3D or 4D datasets. Together with Imaris Track it also allows 4D tracking of all detected structures enabling reporting statistics that change over time for both dendrites and spines including information on counts, branching, size, shape, and intensity.

Imaris

Imaris core is an essential module which delivers all the necessary functionality for experiment data management, visualization and segmentation of the 3D and 4D microscopy datasets. Combining speed, precision and ease-of-use, Imaris provides a complete set of features for working with multi-channel datasets from a few megabytes to multiple gigabytes in size. Multiple volume rendering and modes, clipping planes, cross-section slices will let you create stunning animations and snapshots ready to be used in publications.

Imaris
  • Search, tag, and manage your data
  • Organize and manage full experiments
  • Use premier volume rendering in 3D/4D
  • Detect objects using Spots and Surfaces
  • Enjoy interactive and intuitive workflows
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Filament Tracer

FilamentTracer is the most advanced software product for the automated detection of neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D. FilamentTracer provides a huge selection of Neuroscience specific statistical values: Dendrite Volume, Dendrite Length, Number of Dendrite Segments, Number of Dendrite Branches, Dendrite Area, Spine Volume, Spine Length, Number of Spines, Scholl Analysis, and many more.

Imaris Cell
  • Use versatile tracing methods
  • Obtain branch length and diameter statistics
  • Manually draw segments with automated centering
  • Utilize multiple branch selection methods
  • Visualize filaments along with non-filamentous structures
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Imaris Track

FilamentTracer is the most advanced software product for the automated detection of neurons (dendritic trees, axons and spines), microtubules, and other filament-like structures in 2D, 3D and 4D. FilamentTracer provides a huge selection of Neuroscience specific statistical values: Dendrite Volume, Dendrite Length, Number of Dendrite Segments, Number of Dendrite Branches, Dendrite Area, Spine Volume, Spine Length, Number of Spines, Scholl Analysis, and many more.

Imaris
  • Automatically track objects in 3D + time
  • Choose from a large variety of tracking algorithms
  • Handle thousands of objects and time points
  • Manually edit, create or revise tracks
  • Generate object and track statistics based on changes
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Imaris XT

ImarisXT is a multi-functional two-way interface from Imaris to classic programming languages like Matlab, Python or Java enabling users rapid development and integration of custom algorithms that are specific and tailored to scientific applications where generic image processing would fail. Imaris XT enables free access to pre-existing library of XTensions available on Imaris Open which let you perform, i.e. : relative distance analysis between biological structures, quantifying the object-based colocalization of multiple vesicle markers, visualization and quantification of overlap region and contact area between two 3D structures.

ImarisXT
  • Develop custom algorithms for image processing and segmentation
  • Answer challenging questions with targeted tools
  • Integrate new modules with a customizable user interface
  • Exchange data with Matlab, Java and Python
  • Free access to pre-existing library of XTensions
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Imaris Vantage

Welcome to a new era of data interpretation, plotting and presentation. This module enables you to compare and contrast experimental groups by visualizing your image data (segmented and original) in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker (5-number summary) plots Vantage will help you interpret intrinsically complex and dynamic phenomena. ImarisVantage enables researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects

Imaris Vantage
  • Create fast, explorative and interactive multi-dimensional plots with real objects
  • Define different variable for each of the 5 dimensions (x, y, z, colour, size)
  • Create side by side ready to publish univariate plots to compare groups
  • Export figures and annotations into your presentations
  • Get preview of the results of: Wilcoxon, T-test, F-test and Kolmogorov-Smirnov
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Imaris Coloc

ImarisColoc has been designed to give tools to quantify and document co-distribution of multiple stained biological components in 3D or 4D datasets. ImarisColoc provides an array of statistical parameters that include the number of co-localized voxels, the % of dataset, ROI or channel that is co-localized. More importantly, ImarisColoc offers a choice of well-established co-localization coefficients: Pearson’s and Mander's.

Imaris Coloc
  • Multiple colocalization selection methods
  • Obtain statistics in real time
  • Present data as new 3D or 4D color channel
  • Expand or narrow the computed histogram region
  • Co-localization of entire time series analyzed in fewer steps
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Measurement Pro

Imaris MeasurementPro enables researchers to extract critical statistical parameters from their microscopy images thus allowing for the quantification of scientific findings. Imaris MeasurementPro adds shape, size, and intensity based measurement capabilities to the volume rendering, surface rendering and object detection features of Imaris. It allows researchers to interactively classify, group, and filter segmented objects based on any of the calculated statistics. MeasurementPro enables 3D distance and angle measurements in your dataset.

Measurement Pro
  • Obtain, view and export statistics
  • Obtain precise measurements of intensity values
  • Define object-based coordination system to compensate for translational and rotational object movement during observation
  • Measure distances between points within an object
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Additional Resources

The Imaris Learning Center hosts a wide range of tutorial videos, how-to articles and webinars to guide you through the many features of Imaris. We have provided some links below which will get you started on some of our most recent developments.


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