Imaris Learning: Passive and Whole Body CLARITY for Single Cell Phenotyping

Passive and Whole Body CLARITY for Single Cell Phenotyping

October 2014

Speakers: Dr Benjamin E. Deverman, PhD (Senior Research Scientist, California Institute of Technology) and Arvonn Tully (Advanced Application Scientist, Bitplane)

Understanding the structure-function relationships at cellular, circuit, and organ-wide scale requires 3D anatomical and phenotypical maps, currently unavailable for many organs across species. At the root of this knowledge gap is the absence of a method that enables whole-organ imaging. Recently we presented techniques for tissue clearing in which whole organs and bodies are rendered macromolecule-permeable and optically transparent, thereby exposing their cellular structure with intact connectivity (Yang et al 2014).

Therein we described PACT (passive clarity technique), a protocol for passive tissue clearing and immunostaining of intact organs; RIMS (refractive index matching solution), a mounting media for imaging thick tissue; and PARS (perfusion-assisted agent release in situ), a method for whole-body clearing and immunolabeling. We showed that in rodents PACT, RIMS, and PARS are compatible with endogenous-fluorescence, immunohistochemistry, RNA single-molecule FISH, long-term storage, and microscopy with cellular and subcellular resolution.

These methods are applicable for high-resolution, high-content mapping and phenotyping of normal and pathological elements within intact organs and bodies. In this webinar we will provide an overview of these techniques.

Key learning objectives:

  • Basics and application of a novel whole body tissue clearing technique – PACT (passive clarity technique)
  • Imaris Software as a tool for visualisation and morphology analysis of the structures
Multimedia Library
Application Images (7)
Publications Database
PERINATAL HIGH FAT DIET INCREASES HIPPOCAMPAL VULNERABILITY TO THE ADVERSE EFFECTS OF SUBSEQUENT HIGH-FAT FEEDING
HMGB1 facilitates repair of mitochondrial DNA damage and extends the lifespan of mutant ataxin-1 knock-in mice
3-D Imaging and Analysis of Neurons Infected In Vivo with Toxoplasma gondii
Rapid neurogenesis through transcriptional activation in human stem cells
Novel IL1RAPL1 mutations associated with intellectual disability impair synaptogenesis
Imaging Cleared Intact Biological Systems at a Cellular Level by 3DISCO
Inside Alzheimer brain with CLARITY: senile plaques, neurofibrillary tangles and axons in 3-D
Rax regulates hypothalamic tanycyte differentiation and barrier function in mice
Genetic Manipulation of Cerebellar Granule Neurons In Vitro and In Vivo to Study Neuronal Morphology and Migration
RNA-binding protein Sam68 controls synapse number and local {beta}-actin mRNA metabolism in dendrites
Selective Ablation of Pillar and Deiters' Cells Severely Affects Cochlear Postnatal Development and Hearing in Mice
Enhanced Recruitment of Endosomal Na+/H+ Exchanger NHE6 into Dendritic Spines of Hippocampal Pyramidal Neurons during NMDA Receptor-Dependent Long-Term Potentiation
Relapse Induced by Cues Predicting Cocaine Depends on Rapid, Transient Synaptic Potentiation
Purkinje Cell Ataxin-1 Modulates Climbing Fiber Synaptic Input in Developing and Adult Mouse Cerebellum
Expression of the voltage-gated potassium channel subunit Kv1.1 in embryonic zebrafish Mauthner cell
The Adhesion-GPCR BAI1 Regulates Synaptogenesis by Controlling the Recruitment of the Par3/Tiam1 Polarity Complex to Synaptic Sites
Reinstatement of nicotine seeking is mediated by glutamatergic plasticity
Microglia emerge from erythromyeloid precursors via Pu.1- and Irf8-dependent pathways
Structural and molecular interrogation of intact biological systems
Cerebellar Output in Zebrafish: An Analysis of Spatial Patterns and Topography in Eurydendroid Cell Projections

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