Haeckaliens and OpenT Webinar by Gabriel Martins | Imaris

Haeckaliens and OpenT by Gabriel G. Martins


Speakers: Dr Gabriel G. Martins and Dieter Göhlmann

A standing challenge in developmental biology is the study of morphogenetic processes in vivo and in toto, i.e. inside whole embryos. Conventional microscopy relies on thin tissues, typically 1 to a few cell layers, or 3D imaging of larger tissues, often in vivo, using confocal of 2p microscopy.

However, none of the techniques have allowed deep observation in most mid-to-late stage vertebrate embryos. Two recent techniques have come to aid in imaging both fixed and live thick specimens: optical tomography (aka OPT) and light-sheet microscopy (Sharpe J et al. 2002. Science 296, 541–545; Huisken J et al 2004. Science 305: 1007-1009).

However, commercial solutions are either scarce or non-existent (for OPT), and few prototypical systems are available. Dr Gabriel G. Martins, Advanced Imaging Unit Facility Head, Oeiras, Lisbon, will present a fully open-source, low-cost solution for optical tomography, called OPenT, and show 3D anatomical datasets obtained with large embryos.

The implementation of the system requires only some basic knowledge of optics and electronics using the OpenSPIN microscopy platform (Gualda E et al 2013; Nature Methods 10: 509-510). He will also present an online anatomical database resource for developmental biologists, being prepared with open-source solutions, known currently as “Haeckaliens”.

Key Learning Objectives:

  • Optical Projection Tomography (OPenT) as a non-distructive 3D technique enabling imaging of small species with precise anatomical details.
  • Imaris Software as a powerful visualisation and analytical tool for OPenT images.
Multimedia Library
Application Images (4)
Application Movies (4)
Publications Database
Description of a new syllid species as a model for evolutionary research of reproduction and regeneration in annelids
Indian Hedgehog Mediates Gastrin-Induced Proliferation in Stomach of Adult Mice
Developmental regulation of glial cell phagocytic function during Drosophila embryogenesis
Mouse Fetal Whole Intestine Culture System for Ex Vivo Manipulation of Signaling Pathways and Three-dimensional Live Imaging of Villus Development
The chemokine receptors ACKR2 and CCR2 reciprocally regulate lymphatic vessel density
NMDA receptors on zebrafish Mauthner cells require CaMKII-α for normal development
Segmentation and Quantitative Analysis of Individual Cells in Developmental Tissues
Brain on the stage – Spotlight on nervous system development in zebrafish: EMBO practical course, KIT, Sept. 2013
Modelling cell turnover in a complex tissue during development
The expression analysis of Sfrs10 and Celf4 during mouse retinal development
A novel FoxD3 gene trap line reveals neural crest precursor movement and a role for FoxD3 in their specification
The ETS domain transcriptional repressor Anterior open inhibits MAP kinase and Wingless signaling to couple tracheal cell fate with branch identity
Synchronous and symmetric migration of Drosophila caudal visceral mesoderm cells requires dual input by two FGF ligands
Sox2 and Mitf cross-regulatory interactions consolidate progenitor and melanocyte lineages in the cranial neural crest
Segmental territories along the cardinal veins generate lymph sacs via a ballooning mechanism during embryonic lymphangiogenesis in mice
Preparation and 4D Fluorescent Imaging of Quail Embryos
Primitive Endoderm Differentiates via a Three-Step Mechanism Involving Nanog and RTK Signaling
Nephron formation adopts a novel spatial topology at cessation of nephrogenesi

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