Welcome to a new era of data interpretation, plotting and presentation. This module enables you to compare and contrast experimental groups by visualizing your image data (segmented and original) in five dimensions as uni- or multi-variate scatterplots. Along with the use of box + whisker (5-number summary) plots Vantage will help you interpret intrinsically complex and dynamic phenomena. ImarisVantage enables researchers to dissect their multi-dimensional, multi-object images by creating a series of fully customizable plots for better understanding of hidden relationships and associations between calculated measurements, objects or groups of objects.
ImarisVantage is the perfect tool to explore differences between experimental groups (e.g. control vs test). It allows for the creation of interactive plots which help illustrate relationships/patterns/differences between objects or groups of objects. The users can produce informative and visually pleasing plots in which object data can be shown in multiple scalable dimensions overlaid with the original 3D volume data.
With multiple views at your disposal, you can turn your 3D and 4D data into a dynamic gallery of individual objects. Advanced time plots with Spots, Surfaces, Filaments and Cells can be created to display objects interactively as they undergo temporal changes. Furthermore, you can use ImarisVantage’s scatter plots to produce detailed and customized views of data in four dimensions with additional scaling and color-coding for better understanding of underlying connections between objects. Imaris Vantage also creates single-dimension scatterplots of univariate statistics from one or more image or group of images. This option enables easy, ready to publish comparisons of the distribution (median and quartiles) of a chosen calculated measurement from several groups of images.
All these analytical tools are now ready to use in ImarisVantage and similarly to other functions in Imaris, they use an intuitive Creation Wizard. It guides the user through the step-by-step procedure to create the desired visualization optimizing the final plot and the data presented within it.
In addition to these new powerful data visualization tools, all the data and views created in Imaris can be annotated with individual titles, captions, labels and other highlights for enhancing presentations or publications figures. Both custom text and calculated statistical parameters (require ImarisMeasurement Pro) can be used to label each detected object.
With a few clicks users can add an annotation label to a figure that highlights some detail of the image. Then users can choose which calculated measurement to display. Annotations are fully customizable, you can use different font styles and sizes as well as different styles and colors of arrows and text boxes which is just to mention a few from a range of possibilities for the annotations’ appearance. ImarisVantage allows figures to be exported as an HTML-file, which can easily be opened in most text processors or presentation programs. The image created in ImarisVantage can also be exported by creating a snapshot.
With ImarisVantage you can save the figures with the image data and with the camera or viewing position in 4D space associated for each figure for easy modification if needed.
Imaris Vantage was created having in mind the need to (a) quickly create explorative and interactive single- or multi-dimensional plots and (b) easily compare multiple groups of samples (e.g. control vs test1, test2…) within the Imaris environment.
The values behind all plots created in ImarisVantage can be found in the table underneath the main plot window. You can select objects in 3D space and delve into the statistical underpinnings of your scatter plot or gallery view. There are two tabs available for your convenience, Detailed and Summary (where the 5 number summaries associated with the box + whisker plots is displayed). All values used in plot’s creation process can be exported as a .CSV, .XML or .XLS file for additional processing outside Imaris.
In addition to insightful plots, ImarisVantage allows for the creation of figures and annotations for presentations and publications with just a few clicks. It is as simple as that!
ImarisVantage provides an easy and convenient way to navigate within existing and saved figures. Choose from a range of style possibilities for annotation appearance to adapt output styles to requirements of the publication.
ImarisVantage provides the necessary tools to export figures and plots easily. Both can be seamlessly imported into your favorite word processing or presentation software.
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