Imaris 7.5.0 Release Notes

Welcome to the Imaris 7.5.0 release notes. Version Date: June 13, 2012.
Please have a look to the Overview of Imaris Release notes for information about prior released features and fixed bugs.

[ImarisCell]
[MeasurementPro]
[ImarisXT]
[Imaris]
[Fixed Bugs]
[Feedback]
[Back to Download Page]

ImarisCell
Analysis of membrane stained cells
(no need for cytoplasmic labeling)









  • Brand new fully automated detection algorithm
    (additional and independent from iterative detection algorithm)


    3D embryo (cell membrane labeled). Original image curtesy of Katie McDole,
    Department of Biology, Johns Hopkins University, Baltimore


    2D cells (cell-cell marker). Image on the left from: google images.
    Center and right images are processed in Imaris (Cell).

  • Optimized usability of iterative detection algorithm

    Imaris 7.4.2
     
    Imaris 7.5.0

  • New measurements


    • Full range of cytoplasm-/cytosol-related parameters (e.g. cytoplasm volume and number of vesicles in cytoplasm)
    • Nucleus/Nuclei distance to cell membrane
    • Reporting on different types of vesicles (mean distance of type A vesicle to cell membrane reported separately from mean distance of type B vesicle to cell membrane

  • Variable vesicle diameter within each vesicle type
     

  • New and improved Cell preview and final result rendering

    Imaris 7.4.2Imaris 7.5.0
    Cell preview is grey which allows to visualize the details of the fluorescent signal in a much clearer way than in 7.4.2 when the color or the related channel was used to create the preview of the cell.

    Final rendering is now done in blend mode (90% transparency for the cell, and 0% for the nucleus). The color of the source channel for each component (cell, nucleus and vesicle types) is used to drive the colors of the final rendering.

    This new rendering is particularly advantageous when multiple cells are clustered together. Using MIP only the borders of individual cells cannot be seen. In blend they can. This is even more obvious when moving the scene around in 3D.


MeasurementPro
Statistics
  • Several statistics values have been added for ImarisCell.

ImarisXT
XTensions
  • Improved usability of XTensions to handle Super Resolution Data.
    Added Demo Image and User Guide.




    ... to Spots


    ... to Image


    Volume (detail/zoom)


    Volume + Spots
    The color (and size) of each rendered spot is related to the intensity at the related localization (data from super res. particle table)


Documentation
  • New descriptive library (47 XTensions)

Imaris
Spots
  • Ellipse in Z dimension
  • Mask using spots
  • Exclude when touching image border
Surfaces
  • Bounding box measurements object aligned or axis aligned (L.W.H)
  • Exclude when touching image border
General
  • Customizable / adjustable time stamp configuration and display
  • Color Maps
    • Automatic setting of elucidative default color map (i.e. spectrum)
    • Preview of different color maps
  • Combine multiple Imaris scenes
  • Improved usability for manual object tracking
  • File readers and data exchange
    • Zeiss CZI
    • Improved loading options for iQ users
    • Full metadata data exchange between AQ X3 and Imaris 7.5

Fixed 94 Bugs (since Imaris 7.4.2)
1930Surpass5.0.2Measurment Point labels turn black on some Radeon systems
2948Snapshot / Animation6.1.0Time Bar is corrupt (colour and text) when taking snapshots of tracks
3101Documentation6.1.3Documentation of the XTensions
3426Surpass6.2.1Snapshot messes up position of two colorBars
3429Surpass6.2.1Two colorbars are wrongly rendered in snapshot
3676Surpass6.3.1Rebuild Surfaces Region takes incorrect recreation path.
4223Surpass Cells7.0.0Cells color cannot be changed after switching back from color coding
4320Snapshot / Animation7.0.0Colorbar position not adjustable in snapshot mode with off screen rendering on 64 bit system
4378Surpass Cells7.0.0Cells split by nuclei with nucleus background subtraction produces uneven cells
4450Coloc6.4.2user interface heavily flickering, when playing time animation in coloc
4640Surpass Surfaces7.1.1Key Frame Animation Properties should not overwrite common Object Properties (when loading from file)
4881Surpass Spots7.1.1Automatic region threshold for spots with different sizes (region growing) is always 125
5024Snapshot / Animation7.2.0Conversion Context error when saving a quicktime movie
5251Surpass7.2.3Color of day in timestamp changes if time colorbar hidden
5252Surpass7.2.3hide scalebar: can't move/resize Surpass timestamp anymore
5347XT6.3.1Accessing deleted Spot element from XT crashes Imaris
5366FileIO7.2.3Added channels are turned off in animation
5427License7.3.0RLM license server crashes
5498Snapshot / Animation7.3.0Can not write keyframe movies to folders with French characters
5542_General7.3.0Check for Update does not report new version
5573Surpass Cells7.3.1Long statistic name corrupts colour bar
5610FileIO7.1.1Improper reading of the time data with ND2 files
5625FileIO6.1.5TiffSeries reader crash
5628FileIO7.3.0OpenLabLiff crashes
5642_General6.0.2Resampling Open slice preview not rendering right on Radeon GPU
5707Vantage7.4.0Bottom Part of some Letters in Main toolbar is missing (i.e. "Vantage" reads more like "Vantaqe")
5764Surpass Surfaces6.2.0Edit buttons overlap vertically
5797Vantage7.3.0Colorbar of Surpass stays when switching to Vantage
5820Surpass Volume / Slice7.3.1Shadow causes color gradient on frame shading
5843Surpass Cells7.3.1Vesicles Style Dialog missing, if name of vesicles contains the name "cell"
5854Vantage7.4.0Vantage wizard fails, if some of the key statistics are switched off (e.g. "Position X")
5889Documentation7.4.0Online documentation link for Surface Edit tab broken
5913Surpass7.3.1Colorbar for invisible objects shown after loading
5914Documentation6.4.0Formula for instantaneous Speed Calculation (Spot,Surfaces) wrong in the Reference Manual
5921Surpass Filaments7.4.0For Rebuild Tracks it is not clear that all steps of the wizards will be repeated if 'Entire Image in All Time Points' is selected.
5923Documentation7.4.0Evaluation license description wrong
5958License7.4.0After changing the checked status of the license the table jumps to the top
5967Surpass7.4.0Visibility state of objects not restored when IMS is loaded
5980Surpass Filaments7.4.0Threshold based filaments algorithm does not split unconnected filaments
6008Statistics7.3.0Detailed - Averages Values' statistics export generates corrupt file
6017FileIO7.4.0ICS/IDS with �? character in filename can not be read
6020XT7.4.0XTension Spots Split Into Surface Objects: mixed result for creation wizard status
6024Surpass Volume / Slice7.3.0Using Volume->Draw on newly created channel --> Crash!
6025Statistics7.4.0critically wrong statistic label: Displacement^2 [X Y Z]
6026Time / Tracking7.4.0Time lapse playback gets stuck playing, only force quit can recover
6030Surpass Filaments7.3.1Filament wizard does not show Filament tracking options if only one Filament identified
6034Vantage7.4.0Crash when Nucleus Position Relative to Cell Position is selected for scatterplot
6045Surpass Surfaces7.4.0Set default set vertex delay back to 300 ms
6046XT7.4.1Fiji connection does not work after updating Fiji - Error: Could not create java virtual machine
6048FileIO7.4.0OME Tiff written by Imspector (Lavision Biotech) not detected as OME
6049XT7.4.2Line Profile XTension saves *.csv files with wrong (*.cvs) filename extension
6050XT7.4.0"Licenses currently in use" dialog blocks usage of Imaris via ICE on OSX
6056FileIO7.4.0Bad Metadata.txt (Micromanager format) causes instant crash
6065Documentation7.3.0XT API Documentation: Dead link to ICE on first page
6066Image Edit7.3.0"Apply" button for data-cache in preferences useless
6067FileIO7.3.0Loading speed of OME tiff decreases dramatically while loading (time series)
6070Surpass Surfaces7.4.0after masking surface multiple times, the channel dialog is ignored
6076Surpass7.4.0Threshold setting follows mouse movement
6087FileIO7.4.2OME XML (*.ome) reader loads corrupted voxel data
6088FileIO6.0.1highlighting certain .txt files in Open dialog can crash Imaris
6090Surpass Filaments7.2.0No statistics coded colors for tracks in color tab
6096FileIO7.3.1Segmentation and rendering of large structures fails after the middle of a time series (image dimension: 1061 x 1061 x 10 x 60)
6099XT7.4.2Spots Closest Distance XTension: Border mode fails
6109Surpass Filaments7.3.0Dendrite Beginning Point is removed, if deleting some selection breaks one Filament into multiple unconnected parts
6110Surpass Surfaces7.4.0Bug im Manual Contour: Channel visibility does not work in Surpass Surface
6111XT7.4.0XTSuperResolutionSpotsToDataset missing as matlab runtime for mac
6120XT7.4.0Image To Fiji losses the data for the second channel
6122Surpass7.4.2Crash deleting component with annotation and vantage
6126Surpass7.4.2Rebuilding color coded Spots/Surfaces causes instant Crash
6141XT7.4.0Documentation error in Imaris::IDataItem::GetColorRGBA and SetColorRGBA
6155Surpass Cells7.4.2Cells Settings Tab: Group-box "Vesicle Style / Quality" should not be visible under "Nuclei Tracks"
6156Surpass Cells7.4.2Cells Color Tab: Vesicle tracks are listed by number and not by name
6160Surpass Surfaces7.4.2Manual creation of surfaces crashes on big dataset
6161XT7.4.2Multiresolution images are not recalculated when changing the image data
6164Surpass Cells7.2.3Calculation of Distances to Cell incorrect
6165_General7.4.2Zoom factor after starting Imaris set to -100%
6166Image Edit7.4.2Add [channels/timepoints/slices] fails with multi-image (.SLD, .LIF, .kinetic, etc) files
6169FileIO7.4.2File open failed on second time.
6176License7.4.2In License Administrator the link to the reference manual doesn't work anymore
6184Surpass Cells7.3.0Number of membrane detection iterations is not saved in the creation parameters
6187Surpass7.3.1Crash when renaming component
6189_General7.4.2Copyright year is out of date
6191Surpass Spots7.3.1Using a statistically coded color map during the Spots creation wizard causes a crash
6192XT7.3.0TrackTranslate
6196FileIO7.3.0Ome Tiff load wrong data
6198Vantage7.4.2Imaris crashes with spots/surfaces merge if Vantage object was created on scene object that is no longer present
6216Surpass Filaments7.4.2Rebuild Threshold algorithm 'Keep Data' crash
6220_General7.4.2Crash changing preferences or image origin after deleting Spots/Surfaces
6228FileIO6.4.2Imaris crashes loading surface tracking scene file without dataset
6253Surpass Cells7.0.0Overlapping Vesicles are not removed
6255Surpass Cells7.3.1Crash on drift correction of cells
6258Surpass Cells7.4.2Deleting vesicles does not remove all selected vesicles
6275FileIO7.4.2Some Nikon ND2 version 3.0 files cannot be read
6296FileIO7.4.0Save button does not save manually added surfaces

Feedback
Any feedback on the installation, and the usage of Imaris and its features are welcome. Please send your comments to eusupport@bitplane.com

Last Modification: CL, 13-Jun-2012