ImarisCell
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Analysis of membrane stained cells
(no need for cytoplasmic labeling)




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- Brand new fully automated detection algorithm
(additional and independent from iterative detection algorithm)

3D embryo (cell membrane labeled). Original image curtesy of Katie McDole,
Department of Biology, Johns Hopkins University, Baltimore
  
2D cells (cell-cell marker). Image on the left from: google images.
Center and right images are processed in Imaris (Cell).
- Optimized usability of iterative detection algorithm
 Imaris 7.4.2 |
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 Imaris 7.5.0 |
- New measurements

- Full range of cytoplasm-/cytosol-related parameters (e.g. cytoplasm volume and number of vesicles in cytoplasm)
- Nucleus/Nuclei distance to cell membrane
- Reporting on different types of vesicles (mean distance of type A vesicle to cell membrane reported separately from mean distance of type B vesicle to cell membrane
- Variable vesicle diameter within each vesicle type

- New and improved Cell preview and final result rendering
| Imaris 7.4.2 | Imaris 7.5.0 |
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Cell preview is grey which allows to visualize the details of the fluorescent signal in a much clearer way than in 7.4.2 when the color or the related channel was used to create the preview of the cell.
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Final rendering is now done in blend mode (90% transparency for the cell, and 0% for the nucleus). The color of the source channel for each component (cell, nucleus and vesicle types) is used to drive the colors of the final rendering.
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This new rendering is particularly advantageous when multiple cells are clustered together. Using MIP only the borders of individual cells cannot be seen. In blend they can. This is even more obvious when moving the scene around in 3D.
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Imaris
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Spots
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- Ellipse in Z dimension

- Mask using spots
- Exclude when touching image border
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Surfaces
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- Bounding box measurements object aligned or axis aligned (L.W.H)

- Exclude when touching image border
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General
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- Customizable / adjustable time stamp configuration and display

- Color Maps
- Automatic setting of elucidative default color map (i.e. spectrum)
- Preview of different color maps
- Combine multiple Imaris scenes
- Improved usability for manual object tracking
- File readers and data exchange
- Zeiss CZI
- Improved loading options for iQ users
- Full metadata data exchange between AQ X3 and Imaris 7.5
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| Fixed 94 Bugs (since
Imaris 7.4.2) |
| 1930 | Surpass | 5.0.2 | Measurment Point labels turn black on some Radeon systems |
| 2948 | Snapshot / Animation | 6.1.0 | Time Bar is corrupt (colour and text) when taking snapshots of tracks |
| 3101 | Documentation | 6.1.3 | Documentation of the XTensions |
| 3426 | Surpass | 6.2.1 | Snapshot messes up position of two colorBars |
| 3429 | Surpass | 6.2.1 | Two colorbars are wrongly rendered in snapshot |
| 3676 | Surpass | 6.3.1 | Rebuild Surfaces Region takes incorrect recreation path. |
| 4223 | Surpass Cells | 7.0.0 | Cells color cannot be changed after switching back from color coding |
| 4320 | Snapshot / Animation | 7.0.0 | Colorbar position not adjustable in snapshot mode with off screen rendering on 64 bit system |
| 4378 | Surpass Cells | 7.0.0 | Cells split by nuclei with nucleus background subtraction produces uneven cells |
| 4450 | Coloc | 6.4.2 | user interface heavily flickering, when playing time animation in coloc |
| 4640 | Surpass Surfaces | 7.1.1 | Key Frame Animation Properties should not overwrite common Object Properties (when loading from file) |
| 4881 | Surpass Spots | 7.1.1 | Automatic region threshold for spots with different sizes (region growing) is always 125 |
| 5024 | Snapshot / Animation | 7.2.0 | Conversion Context error when saving a quicktime movie |
| 5251 | Surpass | 7.2.3 | Color of day in timestamp changes if time colorbar hidden |
| 5252 | Surpass | 7.2.3 | hide scalebar: can't move/resize Surpass timestamp anymore |
| 5347 | XT | 6.3.1 | Accessing deleted Spot element from XT crashes Imaris |
| 5366 | FileIO | 7.2.3 | Added channels are turned off in animation |
| 5427 | License | 7.3.0 | RLM license server crashes |
| 5498 | Snapshot / Animation | 7.3.0 | Can not write keyframe movies to folders with French characters |
| 5542 | _General | 7.3.0 | Check for Update does not report new version |
| 5573 | Surpass Cells | 7.3.1 | Long statistic name corrupts colour bar |
| 5610 | FileIO | 7.1.1 | Improper reading of the time data with ND2 files |
| 5625 | FileIO | 6.1.5 | TiffSeries reader crash |
| 5628 | FileIO | 7.3.0 | OpenLabLiff crashes |
| 5642 | _General | 6.0.2 | Resampling Open slice preview not rendering right on Radeon GPU |
| 5707 | Vantage | 7.4.0 | Bottom Part of some Letters in Main toolbar is missing (i.e. "Vantage" reads more like "Vantaqe") |
| 5764 | Surpass Surfaces | 6.2.0 | Edit buttons overlap vertically |
| 5797 | Vantage | 7.3.0 | Colorbar of Surpass stays when switching to Vantage |
| 5820 | Surpass Volume / Slice | 7.3.1 | Shadow causes color gradient on frame shading |
| 5843 | Surpass Cells | 7.3.1 | Vesicles Style Dialog missing, if name of vesicles contains the name "cell" |
| 5854 | Vantage | 7.4.0 | Vantage wizard fails, if some of the key statistics are switched off (e.g. "Position X") |
| 5889 | Documentation | 7.4.0 | Online documentation link for Surface Edit tab broken |
| 5913 | Surpass | 7.3.1 | Colorbar for invisible objects shown after loading |
| 5914 | Documentation | 6.4.0 | Formula for instantaneous Speed Calculation (Spot,Surfaces) wrong in the Reference Manual |
| 5921 | Surpass Filaments | 7.4.0 | For Rebuild Tracks it is not clear that all steps of the wizards will be repeated if 'Entire Image in All Time Points' is selected. |
| 5923 | Documentation | 7.4.0 | Evaluation license description wrong |
| 5958 | License | 7.4.0 | After changing the checked status of the license the table jumps to the top |
| 5967 | Surpass | 7.4.0 | Visibility state of objects not restored when IMS is loaded |
| 5980 | Surpass Filaments | 7.4.0 | Threshold based filaments algorithm does not split unconnected filaments |
| 6008 | Statistics | 7.3.0 | Detailed - Averages Values' statistics export generates corrupt file |
| 6017 | FileIO | 7.4.0 | ICS/IDS with �? character in filename can not be read |
| 6020 | XT | 7.4.0 | XTension Spots Split Into Surface Objects: mixed result for creation wizard status |
| 6024 | Surpass Volume / Slice | 7.3.0 | Using Volume->Draw on newly created channel --> Crash! |
| 6025 | Statistics | 7.4.0 | critically wrong statistic label: Displacement^2 [X Y Z] |
| 6026 | Time / Tracking | 7.4.0 | Time lapse playback gets stuck playing, only force quit can recover |
| 6030 | Surpass Filaments | 7.3.1 | Filament wizard does not show Filament tracking options if only one Filament identified |
| 6034 | Vantage | 7.4.0 | Crash when Nucleus Position Relative to Cell Position is selected for scatterplot |
| 6045 | Surpass Surfaces | 7.4.0 | Set default set vertex delay back to 300 ms |
| 6046 | XT | 7.4.1 | Fiji connection does not work after updating Fiji - Error: Could not create java virtual machine |
| 6048 | FileIO | 7.4.0 | OME Tiff written by Imspector (Lavision Biotech) not detected as OME |
| 6049 | XT | 7.4.2 | Line Profile XTension saves *.csv files with wrong (*.cvs) filename extension |
| 6050 | XT | 7.4.0 | "Licenses currently in use" dialog blocks usage of Imaris via ICE on OSX |
| 6056 | FileIO | 7.4.0 | Bad Metadata.txt (Micromanager format) causes instant crash |
| 6065 | Documentation | 7.3.0 | XT API Documentation: Dead link to ICE on first page |
| 6066 | Image Edit | 7.3.0 | "Apply" button for data-cache in preferences useless |
| 6067 | FileIO | 7.3.0 | Loading speed of OME tiff decreases dramatically while loading (time series) |
| 6070 | Surpass Surfaces | 7.4.0 | after masking surface multiple times, the channel dialog is ignored |
| 6076 | Surpass | 7.4.0 | Threshold setting follows mouse movement |
| 6087 | FileIO | 7.4.2 | OME XML (*.ome) reader loads corrupted voxel data |
| 6088 | FileIO | 6.0.1 | highlighting certain .txt files in Open dialog can crash Imaris |
| 6090 | Surpass Filaments | 7.2.0 | No statistics coded colors for tracks in color tab |
| 6096 | FileIO | 7.3.1 | Segmentation and rendering of large structures fails after the middle of a time series (image dimension: 1061 x 1061 x 10 x 60) |
| 6099 | XT | 7.4.2 | Spots Closest Distance XTension: Border mode fails |
| 6109 | Surpass Filaments | 7.3.0 | Dendrite Beginning Point is removed, if deleting some selection breaks one Filament into multiple unconnected parts |
| 6110 | Surpass Surfaces | 7.4.0 | Bug im Manual Contour: Channel visibility does not work in Surpass Surface |
| 6111 | XT | 7.4.0 | XTSuperResolutionSpotsToDataset missing as matlab runtime for mac |
| 6120 | XT | 7.4.0 | Image To Fiji losses the data for the second channel |
| 6122 | Surpass | 7.4.2 | Crash deleting component with annotation and vantage |
| 6126 | Surpass | 7.4.2 | Rebuilding color coded Spots/Surfaces causes instant Crash |
| 6141 | XT | 7.4.0 | Documentation error in Imaris::IDataItem::GetColorRGBA and SetColorRGBA |
| 6155 | Surpass Cells | 7.4.2 | Cells Settings Tab: Group-box "Vesicle Style / Quality" should not be visible under "Nuclei Tracks" |
| 6156 | Surpass Cells | 7.4.2 | Cells Color Tab: Vesicle tracks are listed by number and not by name |
| 6160 | Surpass Surfaces | 7.4.2 | Manual creation of surfaces crashes on big dataset |
| 6161 | XT | 7.4.2 | Multiresolution images are not recalculated when changing the image data |
| 6164 | Surpass Cells | 7.2.3 | Calculation of Distances to Cell incorrect |
| 6165 | _General | 7.4.2 | Zoom factor after starting Imaris set to -100% |
| 6166 | Image Edit | 7.4.2 | Add [channels/timepoints/slices] fails with multi-image (.SLD, .LIF, .kinetic, etc) files |
| 6169 | FileIO | 7.4.2 | File open failed on second time. |
| 6176 | License | 7.4.2 | In License Administrator the link to the reference manual doesn't work anymore |
| 6184 | Surpass Cells | 7.3.0 | Number of membrane detection iterations is not saved in the creation parameters |
| 6187 | Surpass | 7.3.1 | Crash when renaming component |
| 6189 | _General | 7.4.2 | Copyright year is out of date |
| 6191 | Surpass Spots | 7.3.1 | Using a statistically coded color map during the Spots creation wizard causes a crash |
| 6192 | XT | 7.3.0 | TrackTranslate |
| 6196 | FileIO | 7.3.0 | Ome Tiff load wrong data |
| 6198 | Vantage | 7.4.2 | Imaris crashes with spots/surfaces merge if Vantage object was created on scene object that is no longer present |
| 6216 | Surpass Filaments | 7.4.2 | Rebuild Threshold algorithm 'Keep Data' crash |
| 6220 | _General | 7.4.2 | Crash changing preferences or image origin after deleting Spots/Surfaces |
| 6228 | FileIO | 6.4.2 | Imaris crashes loading surface tracking scene file without dataset |
| 6253 | Surpass Cells | 7.0.0 | Overlapping Vesicles are not removed |
| 6255 | Surpass Cells | 7.3.1 | Crash on drift correction of cells |
| 6258 | Surpass Cells | 7.4.2 | Deleting vesicles does not remove all selected vesicles |
| 6275 | FileIO | 7.4.2 | Some Nikon ND2 version 3.0 files cannot be read |
| 6296 | FileIO | 7.4.0 | Save button does not save manually added surfaces |