Xuv package for Imaris (http://www.xuvtools.org/)

Fast, reliable and automatic 3D alignment of confocal image stacks

 

Xuv tools is a free, open source and fully automated 3D stitching software for confocal microscopy images.  

The goal of XuvTools is to provide tools, that combine multiple microscopic recordings to obtain a larger field of view (“stitching”) and a higher dynamic range (“HDR” recombination), or better resolution (multi view reconstruction) when working with multiple 3D confocal image stacks. XuvTools runs on Windows Linux and Mac computers and supports 32 and 64 bit operating systems (64 bit preferred). 

Documentation


Papers and Talks on XuvTools


You can find our paper on stitching here:

  • M. Emmenlauer, O. Ronneberger, A. Ponti, P. Schwarb, A. Griffa, A. Filippi, R. Nitschke, W. Driever, and H. Burkhardt. XuvTools: free, fast and reliable stitching of large 3D datasets. Journal of Microscopy, 233(1):42–60, 2009

  PDF on Publisher site (free access)
Abstract of the paper

We also presented stitching with XuvTools with the following posters:

  • M. Emmenlauer, O. Ronneberger, H. Burkhardt, A. Ponti, P. Schwarb, R. Nitschke, and W. Driever. XuvTools: Fast and reliable stitching of large 3D datasets. European Light Microscopy Initiative ELMI, 2008

PDF of the Poster

  • M. Emmenlauer and O. Ronneberger and M. Temerinac and H. Burkhardt and A. Filippi and R. Nitschke and W. Driever. Fast Automatic Recombination of Multiple 3D Datasets for Large Volumes and High Dynamic Range (HDR). 49th Symposium of the Society for Histochemistry, 2007

  PDF of the Poster 

  • M. Emmenlauer. Fast, reliable and automatic 3D alignment of confocal image stacks: Practical. UTSA Workshop for Imaging in Neuron Biology, 2010 

  PDF of the Talk

 

XuvTools Cheatsheet - Highly Recommended

A very condensed collection of XuvTools specifics and operation on one page. Includes some notes on microscope setup, and some general facts.

 

Screenshots

 

Xuv Gallery 

 

Worm5 / C.elegans

C.elegans worm from publicly available testdata (by Peter Meister, FMI Basel)

worm5 stitched:


 

sxn3_w1RGB-488nm, sxn3_w2RGB-561nm / Mouse brain

Mouse brain (by Dominique Spirig, FMI Basel)

sxn3_w1RGB-488nm, sxn3_w2RGB-561nm stitched:

 

 

To15100740xm / Neuron filaments

Neuron filaments from publicly available testdata (by Ivan Galimberti, FMI Basel)

To15100740xm stitched:

 
 

Video Tutorials


Video Tutorials - Highly Recommended

The video tutorials are the recommended way to learn how to use XuvTools. Please watch at least one before starting the software.

 

  • Tutorial 1: A Basic Introduction



  • Tutorial 2: A Shorter Introduction



  • Tutorial 3: Stitching with the Layout Tool (Grids)