Welcome to a new era of data interpretation, plotting and presentation. This module enables you to compare/contrast experimental groups with the use of box + whisker (5-number summary) plots. Moreover, you can visualize your image data (segmented and original) in five dimensions and which will help you interpret intrinsically complex and dynamic phenomena. ImarisVantage enables the researchers to dissect their multi-dimensional, multi-object images by creating series of fully customizable plots for better understanding of hidden relationships and associations between calculated parameters, objects or groups of objects. By linking directly to MeasurementPro, ImarisVantage can access its vast array of statistics to annotate objects in the image with the calculated key statistical parameters. These annotations can be placed on and linked to structures in 3/4D which helps prepare the results for publication.
ImarisVantage is the perfect tool to explore differences between experimental groups (e.g. control vs test). It allows for the creations of interactive plots which help illustrate relationships/patterns/differences between objects or groups of objects. The users can produce informative and visually pleasing plots in which object data can be shown in multiple scalable dimensions overlaid with original 3D volume data.
With multiple views at your disposal, you can turn your 3D and 4D data into a dynamic gallery of individual objects. Also advanced time plots with spots, surfaces, filaments and cells can be created to display objects interactively as they undergo temporal changes. Finally, you can use ImarisVantage’s scatter plots to produce very detailed and customized views of data in four dimensions with additional scaling and color-coding for better understanding of underlying connections between objects.
All these analytical tools are now ready to use in ImarisVantage and similarly to other functions in Imaris, they use intuitive Creation Wizard. It guides the user through the step-by-step procedure to create the desired visualization optimizing the final plot and the data presented within.
In addition to these new powerful data visualization tools, all the data and views created in Imaris can be annotated with individual titles, captions, labels and other highlights for enhancing presentations or publications figures. Both custom text and calculated statistical parameters (require ImarisMeasurement Pro) can be used to label each detected object.
With a few clicks users can add an annotation label to a figure that highlights some detail of the image. Then users can choose. Fully customizable, you can use different font styles and sizes as well as different styles and colors of arrows and text boxes just to mention a few from a range of possibilities for annotations appearance. ImarisVantage allows figures to be exported as an HTML-file, which can easily be opened in most text processors or presentation programs. The image created in ImarisVantage can also be exported by creating a snapshot.
With ImarisVantage you can save the figures with the image data and with the camera or viewing position in 4D space associated for each figure for easy modification if needed.
The ImarisVantage functionality requires Imaris:
Imaris Vantage was created having in mind the need to (a) quickly create explorative and interactive multi-dimensional plots and (b) compare groups of samples (e.g. control vs test) within the Imaris environment.
The numbers behind all plots created in ImarisVantage can be found in the table underneath the main plot window. You can select objects in 3D space and delve into the statistical underpinnings of your scatter plot or gallery view. There are two tabs available for your convenience, Detailed and Summary (where the 5 number summary associated with the box + whisker plots is displayed). All values used in plot’s creation process can be exported as a .CSV, .XML or .XLS file for additional processing outside Imaris.
In addition to insightful plots, ImarisVantage allows for the creation of figures and annotations for presentations and publications with just a few clicks. It is as simple as that!
(5) Navigation and choice of styles
ImarisVantage provides an easy and convenient way to navigate within existing and saved figures. Choose from a range of style possibilities for annotation appearance to adapt output styles to requirements of the publication.
ImarisVantage provides the necessary tools to export figures and plots easily. Both can be seamlessly imported into your favorite word processing or presentation software.
ImarisVantage plots and figures could be exported and used outside of Imaris.
The ImarisVantage introduction video tutorial provides an overview of the basic working steps of ImarisVantage plot creation. It contains step-by-step instructions for common work flow of the ImarisVantage plot creation. .
In this video tutorial, we will show you how to customize ImarisVantage plot display. Watch this video and you can learn how to adjust the relative size of the objects, their orientation and position within the plots.