Case Studies
Using Imaris software, the most powerful and versatile 3D and 4D image analysis solution on the market for researchers in life sciences, researchers from Genetech in San Francisco, California, have made new discoveries about the development and function of the blood-brain barrier...
Purkinje neurons are found in the cerebellum and have unique fan-shaped dendrites that align along the functional subdivisions of cerebellar neural circuits. Researchers led by Dr. Mineko Kengaku from RIKEN Brain Institute and Kyoto University in Japan, explored the mechanisms involved in this flat branching by using Imaris software to analyze Purkinje cells in three dimensions...
During gene expression messenger RNA moves from the nucleus to the cytoplasm and although this process has been studied from a biochemical point of view, very little is known about its kinetics. Imaris software helped researchers from Bar-Ilan University in Israel gain in vivo insight by letting them visualize and quantify the kinetics of mRNA transport in single living cells....
Dr. Gary J. Bassell and his lab members from Emory University recently developed an automated method that uses Imaris image processing and analysis software to study dendritic spine morphology. Uncovering the mechanisms regulating dendritic spine morphology is critical for understanding the basic properties of neuronal development and function...
Researchers from Institut Pasteur and INSERM in France, have recently used two photon microscopy and Imaris software to study a mouse model of ear skin transplantation. A better understanding of immune cell migration and the interactions involved in transplant rejection could lead to new ways of controlling the immune response to a transplant...
The naturally circulating hormone estradiol is required for female sexual behavior in rodents, but thus far there hasn’t been evidence that this hormone affects the structure of motivational circuitry. Researchers in Robert L. Meisel’s laboratory at the University of Minnesota recently analyzed dendritic spines with Imaris software....
Chromosomes must establish stable biorientation before anaphase to ensure that both daughter cells share the same genetic information. Researchers led by Jan Ellenberg of the European Molecular Biology Laboratory in Heidelberg, Germany are using Imaris 3D/4D image visualization and analysis software to reveal new information...
As part of the body’s reaction to injury and
infection, leukocytes leave the blood and migrate to the affected
tissue. This migration also plays a significant role in the development
of pathologies where inappropriate inflammation contributes to tissue
damage and disease progression...
Cellular mechanics has been a focus of Dr. Paul Matsudaira’s research for more than a decade. This leading scientist uses and develops powerful microscopy imaging techniques and complex analysis methods to study how cells can carry out complex tasks such as coordinated and three-dimensional movement...
When
the brain is injured, specialized phagocytic microglial cells engulf
and remove cellular debris. However, the role of these cells in
uninjured brains is not clear. Researchers led by Dr. Cornelius Gross
from the European Molecular Biology Laboratory (EMBL) in Italy studied
microglia in uninjured developing mouse brains to find out if these
cells help monitor and maintain synapses...
Hepatitis C viral infection causes chronic liver disease that often leads to liver transplant. However, after a transplant most patients have a recurrence of the virus. In particular, patients with active viral replication process before the transplant often have an increased viral load immediately following liver transplantation...
During clathrin-mediated endocytosis cells selectively internalize molecules from the plasma membrane and surrounding environment. This well-studied pathway allows cells to control the uptake of factors critical for cell growth and proliferation. Consequently, defects in clathrin-mediated endocytosis have been linked to a broad range of diseases such as Alzheimer’s disease, atherosclerosis, and cancer can result...
Streptococcus agalactiae (Group B Streptococcus, GBS) causes serious invasive infections in newborns and adults, particularly in the elderly and individuals with underlying chronic disease. These infections are hard to fight because GBS has evolved several ways to evade the first line of immune defense. GBS can elude macrophages that would normally engulf and kill it, and if engulfed it can even survive inside a macrophage...
There is mounting evidence that protein synthesis and its regulation play a pivotal role in numerous neural processes such as the formation of new synaptic contacts and long-lasting forms of synaptic plasticity. These processes, which are necessary for learning and memory, involve changes in the cellular proteome via protein synthesis, its posttranslational modifications and ultimately degradation...
Developmental biologists seek a better understanding of the methods used by cells to self-organize into appropriate three-dimensional arrangements. Such information could reveal the mechanisms behind certain diseases such as cancers that are characterized by or perhaps even caused by a disruption of tissue architecture. In addition, understanding how cells organize into organs and tissues is necessary for engineering artificial organs and tissues from living cells...
Omid
Khorramshahi, Freie Universität Berlin, and colleagues. Research has
shown that the adhesion proteins known as neuroligins (Nlgs) likely play
an important role in establishing fully functional neuronal circuits.
Mutations in human neuroligin genes have been linked to cognitive
diseases such as autism...
Dr.
E.C. Curnow and colleagues, University of Washington, University of
Sydney and IVF Australia. In vitro oocyte maturation is an established
technique that has been practiced for many years. However, as common as
this practice is, success rates can still be improved. If researchers
can better understand in vitro oocyte saturation, it may lead to better
options for couples with fertility problems...
Dr.
Tomoiku Takaku and colleagues at the National Heart, Lung and Blood
Institute of the NIH. The bone surrounding marrow is very difficult to
cut or image through. However, researchers at the National Institutes of
Health’s National Heart, Lung and Blood Institute have recently
developed a technique to image wholemounts of bone marrow tissue using
3-D confocal fluorescence and confocal reflection microscopy...
Dr.
Coralie Spiegelhalter and colleagues, Institut de Génétique et de
Biologie Moléculaire and other institutions. Researchers at the Institut
de Génétique et de Biologie Moléculaire et Cellulaire in Illkirch,
France have developed a new method for correlating live-cell imaging
with electron microscopy (EM) on cryofixed samples...
Dr. Patricia Barral and colleagues, Cancer Research UK, John Radcliffe
Hospital, University of Birmingham, and Vrije Universiteit. Invariant
natural killer T cells are a subset of T lymphocytes involved in the
host defense against microbial infection. These T cells recognize
glycolipids bound to the surface molecule CD1d, which is expressed by
antigen-presenting cells...
Dr. Willy Suppato colleagues from the California Institute of Technology are using femtosecond laser ablation, fast confocal microscopy and 3D-particle to label, image and quantify fluid flow in zebrafish.
Dr. Tim Worbs, Prof. Reinhold Förster and colleagues from the
Institute of Immunology at Hannover Medical School, are applying intravital two-photon microscopy to investigate the modulatory effects of different chemokines on the basal motility level of T cells. >>>
Prof. Folkesson and Prof. Molin and colleagues from DTU addresse the question of how biofilm organization affects antibiotic susceptibility >>>
Professor R. Klemke and colleagues from UCSD developed a novel translucent zebrafish xenograft model of human cancer progression. >>>